Steam Distillation of Eugenol from Cloves.

Introduction: This experiment involves an extraction of a natural product victimization the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound, which is determine by thin layer chromatography (TLC).

Eugenol is widely utilise in dentistry, due to its analgesic, antiseptic balsamic qualities. It is ideal for curative for treacle hyperemia (the soft, sensitive substance underneath the enamel and dentin of a tooth) and pulp its. In dentistry eugenol is also used to cover cavities, fill canals, etc. It also keeps food honeyed and kills off bacteria and viruses. Eugenol helps keep meat fresh and prevents gestate upset, diarrhea, and wound infections. In addition, it might play a check in relieving indigestion, controlling diarrhea, and healing cuts and bites.

Eugenol is the main active segment in cloves.

Procedure:

1. In a 250 mL round puke flask, place 10.0g of ground cloves and 80 mL of water. Set up for a simple distillation

2. Heat to boiling and collect the distillment until you no longer see droplets of organic liquid advent out of the condenser (about 40 minutes).

3. Place the distillate in a separatory funnel and extract with three 10 mL portions of Dichloromethane

4. Combine the dichloromethane extracts and dry over Sodium Sulfate.

5.



envision the purity of the extract using thin-layer chromatography (TLC). Spot a low sample of the extract using a capillary discoverer about 1 cm from the end of TLC plate.

6. go for using a UV light and mark the imagine spots with a pencil.

7. Record the number of spots and the Rf values from the spot(s).

8. While the plate is developing set up a clean and dry distillation set up and distil off most of the dichloromethane solvent.

9. The remaining resolution of Eugenol in Dichloromethane can be...

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